But Street recognizes total sobriety isn't for everyone.
And Sennett writes admiringly of William Edwards Deming, the twentieth-century business guru who encouraged companies to embrace a program that Pirsig's narrator might recognize: total quality management.
Total protein levels of p47 phox and β-actin in the jejunum of piglets were determined using Abs recognizing total protein.
Phosphorylated and total protein levels of p38, JNK, ERK and β-actin in the jejunum of piglets were determined using Abs recognizing total protein.
Total protein levels of LC3, p62 and β-actin in the jejunum of piglets were determined using Abs recognizing total protein.
Phosphorylated and total protein levels of Akt, mTOR and β-actin in the jejunum of piglets were determined using Abs recognizing total protein.
Similar results were seen using an antibody that recognizes total RNAP (Figure 2C).
To control for equal loading, membranes were stripped when necessary and reprobed with an antibody recognizing total Akt (Akt1/PKBα; Sigma-Aldrich, Steinheim, Germany).
A rabbit polyclonal antibody recognizing both Darpp-32 and t-Darpp (H-62) and a rabbit polyclonal antibody recognizing total Akt/PKB (sc-8312) were purchased from Santa Cruz Biotechnology Santaa Cruz, CA).
In detail, Sepharose columns suitable to capture total immunoglobulins, IgA or IgG fractions were prepared from commercial rabbit antibodies recognizing total human immunoglobulins, heavy IgA or IgG heavy chain (SIGMA-ALDRICH).
Antibodies recognizing total Rb (554136) and p27 (554069) were obtained from BD Transduction Laboratories (San Diego, CA).
