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The isoschizomers HpaII and Msp I recognize and digest CCGG sites, but display differential sensitivity to DNA methylation.
For both assays, we utilized DNases that specifically recognize and digest ssDNA (S1 nuclease) or dsDNA (Shrimp dsDNase).
The essence of this method consists of the ability of the NotI-restriction enzyme to recognize and digest only the unmethylated motif 5′-GCGGCCGC-3′ often found in CpG-islands.
Given that EcoRI was able to recognize and digest γ-HOPdG-modified DNA substrates, γ-HOPdG is presumed to be accommodated in the recognition interface in a manner similar to physiological substrates.
This enzyme recognizes and digests processed, non-primary transcripts that offer a monophosphate at their 5′-end.
The MS-MLPA test utilizes the methylation sensitive enzyme Hha1 (Promega, R6441) which recognizes and digests a CpG site if it is unmethylated [ 30].
Immunity, also termed interference, entails the formation of an endonuclease complex capable of recognizing and digesting invading phage and plasmid DNA.
His ability to read quickly, recognize novel insights, digest new material, and then integrate the knowledge were the hallmarks of his scientific work style.
Pulse field gel electrophoresis was performed on chromosomes derived from purified blood stage parasites and the blot was hybridized against a probe recognizing the HindIII/EcoRV digest tgdhfr/ts fragment obtained from the pBS-TgDHFR vector.
Crazy Good Turns has been off to a strong start and has been featured on the popular podcast The Tim Ferriss Show and been recognized by Podcast Digest as one of the best new podcasts.
In order to observe the time course of ribosylation of lysinyl residures, we used trypsin (which specifically recognizes lysinyl residues) to digest ribosylated α-Syn (Fig. 4A) Ribosylated α-Syn became resistant to trypsin digestion (mass ratio of ribosylated α-Syn to protease: 20∶1).
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