Still other, less widely recognized, cut-offs have been proposed in the literature (9– 11).
Similarly, recognized cut-points can be used for ordinal and continuous variables, such as the rapid walk test [ 15].
Patients' levels of stress related symptoms will be categorized according to recognized cut-off points (See references in Table 1).
As there are no generally recognized cut-off values for the miRNAs analyzed in this work, different values were explored to arrange data (including mean, median and tertiles).
These primary miRNA gene transcripts are typically, but not always, recognized and cut by the endonuclease Drosha in the cell nucleus to produce miRNA hairpin precursors that are then exported to the cytosol, where the hairpin structures are cut by the endonuclease Dicer at relatively fixed positions and released as short double-stranded RNA duplexes [6], [7], [8], [9], [10], [11], [12].
The bead-bound cDNA was subsequently digested with restriction enzyme Nla III, which recognized and cut off the CATG sites.
In the next step this duplex is recognized and cut by RNaseH, resulting in separation of the quencher from the reporter, accompanied by a fluorescence increase.
Such single-stranded 'bubbles' in the PCR product can be recognized and cut by Cel1 or T7E1 nucleases, generating two shorter DNA fragments.
PCR products were digested with specific restriction enzymes that were recognized and cut either at the wild-type or variant sequence site.
To facilitate gene targeting, we designed a pair of TALENs specifically recognized and cut the targeting site of the human GATA2 gene.
Usually, the bead-bound cDNA was subsequently digested with the restriction enzyme NlaIII, which recognized and cut off the CATG sites.
