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Mouse monoclonal antibody against the N-terminus of LRRC25 was used, and the cells used for flow cytometric analysis were not permeabilized, suggesting N-terminus is outside the plasma membrane, and also the antibody recognized a specific staining.
We used the monoclonal antibody against the N-terminus of LRRC25 to perform flow cytometric analysis in ATRA-treated NB4 cells without permeabilization, and found a positive signal only in treated cells (Fig. 3C), suggesting this monoclonal antibody recognized a specific staining.
This finding also precludes the possibility that the antibodies preferentially recognized a specific post-translationally modification or splice variant of the ChePoc1 protein targeted to mitochondria, not produced by the fluorescent-tagged RNA constructs.
While no clear signal was obtained when flower buds were used as starting material (data not shown), the anti-pBRP2 antibody recognized a specific polypeptide of ∼38 kDa in wild-type but not pbrp2-1 pollenextractsn extracts (Figure 4C).
Immunoblotting analysis suggested that antibodies resulting from vaccination with YLP-12 recognized a specific protein band of 72 kDa in testis.
In a similar experiment, anti-Scn1ba_tv2 recognized a specific protein band at ~30 kD in 1610 cells transfected with a scn1ba_tv2 expression plasmid (Fig. 3B, left panel).
Similar(54)
Each factor recognizes a specific DNA sequence next to a gene and sets in motion the machinery that produces the protein specified by the gene's information.
In antisense technology, a short series of nucleotides recognizes a specific sequence on an mRNA strand, preventing it from being translated into a protein and blocking expression of a gene.
These enzymes not only recognize a specific region in a DNA sequence but always cut the DNA at that very site.
The first step is to recognize a specific sequence on DNA called a promoter that signifies the start of the gene.
The antigen-binding site is what allows the antibody to recognize a specific part of the antigen (the epitope, or antigenic determinant).
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