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The monoclonal ANTI-FLAG®M2-FITC (Sigma-Aldrich: F4049), which is a monoclonal antibody covalently conjugated to fluorescein isothiocyanate (FITC), can recognize the FLAG sequence at the N-terminus (Hanson et al., 2007).
"'The Dukes of Hazzard' was popular on TV in Argentina in the '80s, so I'm sure a lot of older people recognize the flag from that, but they don't recognize its meaning in the larger context of U.S. racial politics, or how it's been used as a racist symbol".
Although your kids may easily recognize the flag, it's possible that they may not understand the meaning of the stars, stripes, and colors.
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Since both antibodies recognize the Flag-tagged AR specifically (Figure 2B), we conducted immunostaining assays with anti-Flag (Figure 3A) and anti-AR (Figure 3B) of AR cells with or without R1881 treatment.
The identity of the platypus recombinant MLP was further confirmed by western blot analysis using a mouse anti-DDK to recognize the FLAG-tag fusion MLP with an apparent molecular mass of approximately 49 kDa in the conditioned media and affinity purified samples (fig. 5 B).
As a Revolutionary War re-enactor and American flag buff, Mr. Laube recognized the flag wadded up in the quilt.
The national parliament recognized the flag on March 11 , 1971 and its usage was extended to ships registered in Papua New Guinea when the country became independent on September 16 , 1975
"The mayor recognizes the flag has become an iconic image of America's resilience and has asked the Fire Department to see if it can track the chain of custody," said Edward Skyler, a spokesman for Mr. Bloomberg.
The membranes were probed with an antibody recognizing the FLAG tag (clone M2; Sigma) and anti-mouse-HRP (Sigma).
At 24 h after transfection, we examined the expression of each Tax protein by western blotting using monoclonal antibody (MAb) M2, which recognizes the Flag tag.
At 24 h post transfection, the expression of Tax protein was assessed by immunoblot analysis of cell extracts using the monoclonal antibody (MAb) M2, which recognizes the Flag tag.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com