Sentence examples for recognition restriction from inspiring English sources

Basically, chromatin is cross-linked, followed by digestion with a 6 bp recognition restriction endonuclease, ligation in diluted conditions and decross-linking of the DNA.

The library is subsequently digested with a frequently-cutting 4 bp recognition restriction endonuclease or mechanically sheared to obtain small fragments containing the ligation site, followed by end-repair and ligation of an adapter.

It was not known whether substrate recognition restrictions were similar for forms of UPPS from different bacteria or whether this compound could be used for continuous fluorescent monitoring of UPPS activity.

The bet is that at least four justices (Ginsburg, Breyer, Sotomayor, and Kagan) will strike it down on equal-protection grounds, and one or two (Kennedy, maybe Roberts) more will join them on federalism grounds, leaving perhaps a somewhat muddled precedent, but one bringing the end of federal non-recognition restrictions nonetheless.

Virus discovery cDNA-AFLP (VIDISCA) is a virus discovery method based on recognition of restriction enzyme cleavage sites, ligation of adaptors and subsequent amplification by PCR.

Splitting samples into three reactions and digesting each reaction with one of three 4-base recognition sequence restriction enzymes allowed fragmentation of DNA while maintaining high representation of genome sequences in fragments > 100 bp.

This is a virus discovery method based on recognition of restriction enzyme cleavage sites, ligation of adaptors, and subsequent amplification by PCR combined with high-throughput sequencing 454 FLX/Titanium system (Roche, USA) [ 5].

Expectations about the quality of life were varied, with recognition that restrictions may be based on parental beliefs about asthma, rather than asthma itself.

SNPs were screened for substitutions that altered the recognition sequence of restriction enzymes using the Bio::Restriction::Analysis library of BioPerl [ 33].

The primary function of methylation is DNA self-recognition via restriction-modification systems that protect the organism against invading DNA.

The overbreadth doctrine derives from the recognition that unconstitutional restriction of expression may deter protected speech by parties not before the court and thereby escape judicial review.