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Briefly, each sample (1.5 μg DNA per sample for parent libraries/0.25 μg DNA per sample for offspring libraries) was digested with SbfI-HF (NEB) (recognition cut site 5′ CCTGCA/GG 3′ and 3′ GG/ACGTCC 5′).
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Brand recognition cuts both ways.
Grown up, he goes to him at a concert; then, seeing only dim recognition, he cuts himself with a smashed bottle.
Lacking official recognition, they are cut off from the social services and legal protections that are crucial to their survival and future ambitions.
Lacking official recognition, they are cut off from the social services and legal protections that are crucial to their survival and prospects.
To determine which AsiSI recognition sequences are cut in U2OS cells, we performed ChIP followed by high-throughput sequencing using anti-γH2AX antibody on cells either treated or not with tamoxifen.
But now plenty of companies are finding smart ways to apply voice-recognition technology to cut costs and improve services.
An individual repeat unit of the array is approximately 3.4 kb and is defined by a BglII restriction enzyme recognition site that cuts once per monomer.
Mr. Jeffords's position is a recognition that the tax cut leaves insufficient room for vital domestic needs, defense spending and paying down the debt.
Several Democrats accused the board's majority of hypocrisy because on the same day it decided this case it issued another ruling that allowed a company to cut off recognition of its union after a majority of workers submitted a petition seeking a vote to get rid of it.
Streep expressed strong interest in the role of Susan Orlean before being cast, and took a salary cut in recognition of the film's budget.
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