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The M.EcoKI MTase recognises the sequence A ACNNNNNNG TGC and the methylation status of the adenines at the underline locations.
MspJI is a recently characterised restriction enzyme which recognises the sequence motif CNNR (where R = G or A) when the first base is modified to 5-methylcytosine or 5-hydroxymethylcytosine.
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The trimeric methyltransferase (M2S) recognises the asymmetric sequence (GAAN6RTCG).
An engineered R M system, denoted EcoR124INT, has two copies of the N-terminal domain of the HsdS subunit of EcoR124I, instead of a single S subunit with two domains, and recognises the symmetrical sequence GAAN7TTC.
The enzyme recognises the CGCG sequence and reacts at 60 °C.
NF-κB recognises the consensus sequence GGGRNTYYCC [42].
Kaiso also recognises the consensus sequence TCCTGCNA (N being any nucleotide) throughout a methylated DNA-binding domain independent region.
A generic primer that recognises the adapter sequence was then used to amplify the adapter-ligated fragments in a PCR reaction.
The 5' end of the cDNA encoding omTRPC1 was amplified using the omTRPC1 5'Race primer (5'-CAGGTCTCTCATCTCGTTGCGGAACTT-3') and Universal Primer A Mix (UPM) which recognises the BD SMART sequence.
Purified PCR products (192 bp) were digested with restriction enzyme TaqI (Invitrogen), which recognises the TCGA palindromic sequence unique to the bisulphite-converted DNA of the methylated alleles of the Inr site.
Mash1 is expressed in neural precursors in vertebrates (Parras et al, 2004), is known to regulate genes involved in Notch signalling (Delta, Jag2, Lfng and Magi1), cell-cycle control (Cdc25b) and neuronal differentiation (Insm1) (Castro et al, 2006) and recognises the E-box sequence, CAGCTG (Hu et al, 2004; Castro et al, 2006).
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