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Affinity-purified antibodies recognised the expected size of the endogenous SNX5 (∼ 50 kDa) and also a ∼ 76 kDa band which corresponded to the size of GFP-SNX5 (supplementary material Fig. S3B).
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In contrast, the presence of H2d could be unambiguously recognised, and at the expected position, in the deduced PmCOE-B amino acid sequence (see Additional file 3).
The Myc antibody readily recognised one protein of the expected size in the above cell line induced to express the transgenic protein (data not shown) and also in transiently transfected cells (Figure 2 A) but did not reveal any specific bands in non-induced or control cells validating our Tet-inducible expression system.
Ali and he formed an unlikely friendship, as two black men who recognised the roles a racist country expected them to play and subverted them in different ways.
The affinity-purified antibody (see methods in the supplementary material) recognised a single band of the expected size at 21.5 kDa on western blots from Arabidopsis seedling protein extracts (see methods in the supplementary material), and we detected EYFP-ROP2 and GFP-ROP6 in lines expressing these proteins (supplementary material Fig. S5).
Patterns of profit and investment are changing fast, as people recognise the impossibility of expecting limitless returns from limited resources in our world.
Any day now the first 10 recognised refugees are expected to move out of detention and into the $137m village Australia has built for them in Lorengau, the provincial capital.
I recognised the driver.
But the United States, Colombia, Peru and Costa Rica all recognised the election.
They recognised the voice.
Sure, he recognised the problems.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com