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The flow cytometry data presented here demonstrates that the ribosomal protein L25 (RplY) is available at the cell surface with levels of fluorescence comparable to those found with recognised surface antigens such as NMB1468 [49], NMB2132 and NMB2091 [5], although considerably lower than other surface antigens such as fHBP [5].
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This kit uses a Chlamydiaceae-specific monoclonal fluorescent antibody recognising surface LPS from all known chlamydial species.
Antibodies recognising surface exposed epitopes of OmpA protein could neutralize chlamydial infection both in cell culture and in a mouse animal model [ 13– 16], while antibodies against chaperones correlated with development of infections [ 10, 12, 17– 20].
Bousema, P. Schneider and R. Sauerwein, personal communication) strongly recognised the surface of erythrocytes infected with stage V gametocytes, but did not recognise the surface of erythrocytes infected with 3D7 asexual parasites (Fig. 2).
Anyone familiar with Microsoft's other Surface tablets will instantly recognise the Surface 3. It looks just like the Surface RT and Surface 2 before it, with a grey metal body, three-position kickstand and a capacitive Windows button.
It is recognised that surface topography is the one of the most important factors affecting the functional performance of components.
None of the plasma recognised the surface of erythrocytes infected with developmental stages of gametocytes (I IV), but 66 of 194 (34.0%) plasma contained IgG that recognised the surface of erythrocytes infected with mature (stage V) gametocytes.
Fifty-three (26.4%) of the 200 plasma successfully tested recognised the surface of erythrocytes infected with asexual parasites.
One of the unexpected findings of our study is that none of the sera or plasma tested recognised the surface of erythrocytes harbouring immature stages of 3D7 gametocytes.
However, this serum strongly recognised the surface of erythrocytes infected with mature stage V gametocytes (Fig. 2), suggesting the presence of GSA in the form of parasite-encoded molecules, altered host membrane components, or both on the surface of these cells.
Cross-absorption analysis showed that each antiserum contained antibodies which recognised cell surface antigens preferentially expressed by the donor cell line.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com