Some of the genes (i.e. APC, ATM, BRCA2, CHFR, CDH13, CDKN1B, CDKN2A, CDKN2B, ESR1, FHIT, GSTP1, HIC1, MLH1, PTEN, RARβ, RASSF1A, STK11, TP73, CADM1 (previously referred to as TSLC1), VHL) were represented by two or three probes that each recognised a different Hha1 restriction site in the promoter region of the respective genes.
Accessory sortases generally differ in specificity, recognising a different sortase recognition motif, and their genes tend to be co-located with their substrate proteins.
"Much of the work we do is to get big incumbents to recognise a different future," says John Elkington of SustainAbility, a consultancy.What might the next wave of trouble be?
Finally, p53 expression was measured with an antibody recognising a different epitope of p53.
In order to assess p53 immunoreactivity using an antibody recognising a different epitope of p53, a second antibody was employed, D0-1 (Oncogene Science, Manhasset, NY, USA) 31 cases.
Thus, the use of EDIAs, which recognise a different site on ErbB2, could prove to be effective for treatment of breast cancer that cannot be cured with trastuzumab.
It is likely that it retains DNA-binding activity, but given the lack of conservation of the relevant residues, it will probably either recognise a different sequence from GATA1 or, perhaps more likely, may bind to DNA without sequence specificity.
However, in all cases, we observed a similar signal also in p53-negative mESCs, indicating that these antibodies recognised a protein different from p53.
In industry, he continues, "your success as a researcher may be recognised in a different way, such as being associated with a drug launch," notes James.
It is possible that the ring structure of proline might allow the substrate to be recognised in a different orientation within the PAT2 binding site or even in distinct orientations (as both an α- and a δ-imino acid) which might help explain why both d-proline and trans-4-hydroxy l-proline have similar affinities for PAT2 to that observed with l-proline [26,27,37,38].
Its binding specificity has been evolved to recognise a quite different target, a human monoclonal IgG4.
