These cell surface HLA-B27 homodimers can be recognised by NK receptors such as KIR3DL2 that do not recognise the monomeric form [ 11].
Considering that the K63-linked polyubiquitin chains do not trigger proteasomal degradation of PCNA (Zhao and Ulrich 2010), identification of proteins that recognise the polyubiquitylated form of PCNA might prove crucial to unravel the mechanism of error-free damage bypass.
However, there are no available antibodies that recognise the phosphorylated form of VEGFR2 for flow cytometry and the expression of PDGFR-B is below the detection limit of this technique in PBMCs.
The presence of phosphorylated Cx43 in the Triton insoluble fraction was determined by immunoblotting using polyclonal antibodies that recognise the phosphorylated forms of Cx43.
Although the asparagine in the ligand is N-glycosylated inside ER, the authors find that env-specific CTL clones only recognise the non-glycosylated form.
To investigate the different stages of Src kinase activation, Clone28 antibody (Invitrogen, Paisley, UK) was employed to recognise the partially activated form of Src, as Clone28 recognised Src that is unphosphorylated at Y530, independent of the phosphorylation status of Y419.
At present, the best indication of PI3K activation (by any means), or PTEN loss, is probably to screen tumours with antibodies that recognise the phosphorylated (active) form of Akt.
Blots were probed with human Topo I antibody (TopoGEN, Inc., Columbus, OH, USA) and monoclonal antibodies 8D2, which recognise the Mr 170 000 form of the Topo II α enzyme (kindly provided by Dr A Kikuchi, Laboratory of Medical Micology, Research Institute of Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya).
For this, we have to recognise the millions of forms that tumbled out of this treasure chest called Shakespeare".
