The most powerful tool for site-specific recombination in vitro [10], [11] and in vivo [12], [13] is Cre recombinase, which catalyzes reciprocal site-specific recombination between two loxP sites.
Following introductory workshops the multidisciplinary teams within each pair undertook facilitated reciprocal site visits.
We were not able to determine the breakpoint at the reciprocal site of the translocation.
(H ) A comparison of the percentage of inputs from the reciprocal site (defined by region of BFP infection) of each injection (in red) with the average percentage of inputs from that region among all other brains (in black).
In a detailed investigation of the PaTPS-LAS and PaTPS-Iso enzymes, using reciprocal site-directed mutagenesis and domain-swapping, we have recently shown that four amino acid residues determine the different product profiles of these Norway spruce diterpene synthases [ 24].
It provides, at last, a rational explanation for these properties in light of a proposed bivalent receptor binding model and, via comparison of the data obtained with those known for other mutated analogues, reduced the number of candidate substitutions to a small number that was tested by reciprocal site-directed mutagenesis.
The experimental schemes allowed two cross sections of reciprocal lattice sites to be obtained: normally (ω-scanning) and in parallel (ω/2θ-scanning) to the diffraction vector.
All of the reciprocal garden sites are situated at approximately the same latitude and within USDA hardiness zone 5 (−28.8 to −23.4°C average annual minimum temperature).
Allostery can be defined as a change in binding (k-type response) or catalytic (v-type response) properties of a biopolymer at one site by a perturbation at a distinct site, due to reciprocal energetic coupling between the two events (25).
To evaluate this hypothesis, structures of reciprocal single-site mutants at these 15 positions around the active site of BSP165 and TF MAN in complex with mannotriose were constructed and pKa calculations were carried out (Table 4).
The reciprocal binding site for Dcn1 on Cdc53 is located ∼18 Å from the site of neddylation.
