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Our results provide proof-of-concept that mixed chimerism and tolerance can be attained without toxic recipient preparation.
Its unparalleled effectiveness in achieving robust tolerance is counterbalanced by the toxicity of the drastic cytoreductive recipient preparation necessary for BM engraftment.
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Recipient site preparation included small perforations with spherical low-speed drill and adequate irrigation in order to promote the revascularization of the onlay grafts and the closest adaptation possible.
Among the various parameters influencing the outcome of SCNT, such as oocyte quality, nuclear transfer protocol, embryo culture or recipient animal preparation, the type and quality of the nuclear donor cells are of vital importance (reviewed in [ 11]).
The large sample size of the current study, together with the standardization of the donor stimulation protocols, the recipient endometrial preparation and the sperm sample evaluation provides enough scientific weight to conclude that paternal age does not play a significant role in determining the results of ICSI when the oocyte is young and healthy.
Federal statutes require that welfare recipients prepare for work by engaging in narrowly-defined work activities (unsubsidized employment, job preparation classes, job training).
One means to strengthen regulatory responses is treating recipients with preparations of regulatory cells obtained by ex vivo manipulation.
Cells of C. merolae were transformed by the PEG-method according to Ohnuma et al. (2008) except for the preparation of recipient cells.
Despite protocol modifications in donor selection, islet preparation, or recipient treatment, insulin independence with adequate metabolic control was, however, rarely prolonged beyond 2 years (1– 5).
An argument in favor of affirmative action in college admissions is that it identifies students with much potential but weak preparation, suggesting recipients should catch up to their more-prepared counterparts over time.
The synchronization of the donor stimulation with the endometrial preparation of the recipient is usually done by downregulating the recipient's pituitary with a GnRH analog.
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