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We hypothesized that the bioengineered ring constructs would retain cellular viability and promote neovascularization upon implantation into a recipient mouse.
Therefore, we tested several different combinations of donor and recipient mouse strains as well as the time span for CAF development.
Accordingly, alveoli regeneration by SOX9+ BC transplantation also improved the recipient mouse pulmonary function as shown by the decrease of CO2 partial pressure, increase of O2 partial pressure and O2 saturation in artery blood (Fig. 5C E).
However, spermatozoa from some strains, including C57BL/6 (B6), are very sensitive to freezing and thawing and frequently fail to fertilize eggs by conventional in vitro fertilization methods at the recipient mouse facility.
into individual recipient mouse in different groups.
At the shaved area, 1 cm2 of skin was removed in each recipient mouse.
Recipient (mouse) CD45, but not recipient CD14 or CD41a, could be transferred to donor (human) cells.
Cells recovered from one single primary host were transferred into one secondary recipient mouse.
Each recipient mouse received 3.5×106 activated lymph node cells by i.p. injection.
The donor pig is black and haired, while the recipient mouse is white and hairless.
Each secondary recipient mouse was reconstituted with a fraction of enriched cells equivalent to those from 1 femur.
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