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Formation depends on lymphotoxin-expressing donor cells, whereas further organization requires lymphotoxin-expressing recipient cells.
However, this process is not always effective due to a lack of reaction by recipient cells.
Exosome-shuttled molecules maintain their biological activity and are capable of modulating and reprogramming recipient cells.
Our data show an improvement of transformation frequency by optimizing AMT conditions according to the recipient cells, and which supports that AMT means can be applicable to transform variety types of the recipient cells.
Beyond above pathological effects, EV-associated miRNAs can remarkably change the response of recipient cells to various anticancer agents.
Equally significant, those RNAs could serve as intracellular messengers, inducing recipient cells to change their behavior.
The recipient cells were then recovered from the filter and plated on LB agar plates containing two antibiotics to select recipient cells that had acquired plasmids.
Therefore, the activities of other CAG strains as recipient cells were examined (Table 7).
We then investigated the ability of ESMVs to transfer GFP to recipient cells.
Asc10 expression on the donor cell surface promotes binding to recipient cells.
Mature DCs readily transmitted both HIVBal and RT-SHIV to the recipient cells.
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