Sentence examples for recipient bacteria from inspiring English sources
Plasmid DNA isolated from selected colonies was used to electroporate appropriate recipient bacteria and plated on LB agar plates containing an antibiotic (20 μg/ml of chloramphenicol).
After conjugation of pABRW, in either E. coli SXT (its specific target) or E. coli MG1655 (not targeted) the number of surviving recipient bacteria (c.f.u./ml) was determined by plating on MH media without antibiotic and with arabinose (0,2%).
The first requirement for HGT to occur is the contact between donor cells or their DNA and the recipient bacteria.
The horizontally acquired chimeric elements can provide an immediate selective advantage to recipient bacteria, for example by conferring antibiotic resistance.
However, we were able to demonstrate that the sequences corresponding to resistance genes detected in phage DNA can confer resistance to a recipient bacteria.
Donor and recipient bacteria were then harvested by centrifugation (6000×g, 10 min, 4°C) and 108 of each were mixed and spotted onto BHI agar.
The components of the Mpf system, comprising a minimal set of 10 conserved proteins, form a membrane-spanning protein complex and a surface-exposed sex pilus, which both serve to establish intimate physical contacts with a recipient bacterium.
Natural bacterial transformation involves the transfer of DNA from a donor bacterium to a recipient bacterium, where both donor and recipient are ordinarily of the same species.
Adjacent to the conjugation region is the "transfer leading region", which is initially transferred into the recipient bacterium during the conjugation process [19].
Their stability and effect on the recipient bacterium, especially its pathogenic character, is now a matter of speculation and further research.
During bacterial conjugation, a donor bacterium intimately contacts a recipient to form a conjugative pore (a bacterial type IV secretion system) through which a single-stranded DNA is transferred from the donor to the recipient bacterium.
