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In the initial step, one round of backcrossing is conducted to donate the favorable QTL allele to the recipient background using marker-assisted backcrossing (MAB).
In the case of traits like drought tolerance, where gene-linked markers are not available, the identification of peak markers and flanking markers in the recipient background within the QTL region is necessary at the initiation of a MAB program.
2×10 spleen cells (recipient background) were co-injected to promote short-term survival.
Two homozygous offspring M1M1 were bred to fix the M1 donor marker allele (along with a cosegregating locus of interest) in the recipient background.
P. brassicae GFP-transformant 155m [ 17] – which has HH as recipient background – was kindly provided by Dr. F. Mauch, University of Fribourg, Switzerland.
None of the BnTTG1 over-expression or knockdown lines in the semi-glabrous leaf Westar recipient background showed any phenotypic differences with respect to plant morphology, trichome coverage, growth or reproduction when compared to Westar.
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Validation of candidate genes will thus depend on comparative genomics among a set of relevant indica and aus references and transgenic approaches that knock out the R-alleles in the donor background or transfer the R-alleles into recipient backgrounds.
The allele-specific gene-tagged markers for the target genes are more effective than the genomic random markers surrounding the target gene (from several kb to a few Mb distance) because some markers will not show polymorphism in some recipient backgrounds and sometimes a false-positive allele can be selected by recombination between the target gene and the genomic random marker.
First, cell fusion was initially confirmed in all experimental groups and in all recipient backgrounds used in the studies reported here.
The second reason is the quantitative nature of multiple gene/QTL may partially regulated by recipient genetic background of CholSub1 donor and the 'PinK3', the highly susceptible recipient.
Successful quantitative trait locus (QTL) introgression depends on the optimized expression of newly introgressed QTLs in the recipient genome background, with the aim of maximizing productivity.
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