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Not only did the recipient animals grow stronger skeletons, but their bones regained the healthy coral-like appearance that osteoporosis destroys.
Table 3 shows functional respiratory parameters in the donor and recipient animals.
The current study used dual-modality imaging with PCSN probes labeled with 99mTc and NIR fluorescence to evaluate sentinel lymph nodes in the recipient animals.
For the in vivo phase, flaps were isotransplanted to 35 recipient animals, divided into three groups: fresh (n = 10), perfused (n = 8), and cryopreserved (n = 17).
In a xenotransplantation model using NOD/SCID mice, we found that transduced CD34+ cells could repopulate irradiated recipient animals, as measured by CD25 expression.
Finally, using optical mapping and electrophysiological analyses, we provide evidence of permanent AV conduction through the implant in one-third of recipient animals.
MSCs were harvested from male mice, and male to female transplantation was performed to identify male donor cells within female recipient animals.
One month post-injection, we found specific expression of luciferase in hearts of recipient animals when compared with transgene expression driven by the standard EF1-α promoter.
After washing 3 times, the embryos were transferred into recipient animals.
2×104 cells of each population were injected i.v. into the tail vein of recipient animals.
Cells (∼106) were intravenously injected into lethally-irradiated (11 Gy) recipient animals.
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