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Strains containing plasmid pSU5024 [ 6], which overproduces Eex_R388, were used as controls of poor recipient ability.
However, recipient ability was restored when the conjugation experiments were carried out in surface mating conditions (Fig. 6A).
Under surface mating conditions, the 11 LPS mutants showed similar ALU values with respect to a wt recipient (Fig. 6A) indicating that recipient ability was unaffected.
There is the potential doubt of whether these cells will be amenable to genetic manipulation, perhaps because they lose genes important for recipient ability in conjugation.
The two mutant sets used in this study point out LPS genes as the main non-essential recipient functions implicated in R388 conjugation recipient ability.
To evaluate the recipient ability of our LPS mutants, along with the gppA mutant (130F12), in F plasmid conjugation, a lux derivative of pOX38 was constructed (See Methods).
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