The t1 parameter sets the turnover rate of the CLV1 receptor, including the degradation of the bound receptor (Methods).
In order to begin to study the structural and functional mechanisms of olfactory receptors, methods for milligram-scale purification are required.
Thus, the assay introduced in this study is a nonradioactive receptor binding method that shows promise as a high throughput screening method for large-scale testing of environmental and industrial chemicals for ER binding interactions.
For each hormone receptor assessment method, the continuous logarithmic values were converted to statistically standardized z-scores using the assessment method mean and SD of logarithmic values: z- score = ((log value - mean of log values)÷SD of log values), which has approximately a standard normal distribution, N 0,1).
The hormone receptor scoring method used herein is not finely calibrated, which may affect assay sensitivity; however, a less sensitive scoring method may be more likely to detect only cases with more robust (and, likely, more biological important) expression of these receptors.
Additionally, the aptamer-assisted receptor immobilization method has great potential to become a universal protein immobilization technique in biosensing, and be applied into the development of OR-based biosensor array for the simultaneous detection of several odorants.
It could also be applied for the determination of the dissociation constant of analytes using the previous reported multiple receptor CMSP method.
This receptor binding method was adapted from previous studies (Jones and Wonnacott, 2004; Oddo et al., 2005; Shelukhina et al., 2009).
However, the data may be biased, because the estrogen receptor assay method had changed (ligand-binding assay was used in 1982 to 1986; immunohistochemistry on frozen sections in 1989 to 1992; immunohistochemistry on paraffin sections in 2004 to 2005).
Using generalized log linear regression analysis, we compared age-specific incidence rates with respect to period, stage, oestrogen receptor status, method of detection and use of hormone replacement therapy.
