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Although recent sequencing technologies have greatly improved sequencing throughput, these new technologies introduce multiple errors that are absent from WGS sequencing.
Recent sequencing technologies such as that from 454 Life Sciences have made it possible to quickly and cheaply generate a large number of short clonal transcripts (~100 400 bp).
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A more recent sequencing technology is pyrosequencing, which is based on the principle that nucleotide incorporation in a DNA strand is accompanied by release of pyrophosphate.
Recent sequencing technology has led to a precise understanding of the sequence structure of the human genome, and revealed the presence of copy-number variations (CNVs), which are associated with susceptibility to common diseases [ 73, 74].
In this study, we utilized the most recent sequencing technology available on the Illumina platform to conduct whole transcriptome sequencing of seed tissue from three soybean lines developed in our laboratory.
In recent times, sequencing technologies are becoming increasingly used, both by the university and the industry sectors.
In recent years, sequencing technologies have rapidly evolved from classical Sanger sequencing to NGS [ 70].
In this review, we focus on the disease-specific gene mutations in MLN discovered by recent massive sequencing technologies.
In conclusion, we successfully identified the causative mutation for a very rare autosomal recessive mutation with only one case by exploiting the most recent DNA sequencing technologies.
With the more recent DNA sequencing technologies that produce millions of reads there is increasing need for a scaffold to which these reads can be aligned.
Recent deep sequencing technologies are currently generating data with high coverage (>50-fold on average) irrespective of the organism under study.
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