The absorbance of each well was measured at 405 nm, background signal from the blank wells was subtracted from experimental wells, and the percent decrease in biofilm was calculated by normalizing the signal from enzyme treated wells to that of wells receiving no enzyme treatment.
For each time-point, an aliquot receiving no enzyme was treated in parallel as a control.
Control groups (drug only, light only or both) received no radical scavengers or enzyme inhibitors.
In the present experiment no accumulation of cellobiose was observed, even in the assays receiving no fresh enzymes upon recycle, indicating that β-glucosidase was potentially bound to the solid fraction.
The experimental groups were as follows, Group 1: received no Eimeria oocysts (negative control), Group 2: received oocysts of mixed Eimeria species on day 15 to create an experimental coccidiosis (positive control), Group 3: negative control received phytase enzyme in their diet, from the first day of life, and Group 4: positive control received phytase enzyme in the diet.
No participant received angiotensin-converting enzyme inhibitors/angiotensin receptor blockers at study baseline.
The study, published in The New England Journal of Medicine, found that patients who received the enzyme were less likely to die from a subsequent heart attack.
The samples received from enzyme purification and subsequent buffer exchanges were used without prior dilution, but protein concentration was determined to calculate enzyme activities.
When the researchers cut out the animals' tumors, they were soft and pink, unlike the hard, white tumors from animals that had not received the enzyme, Hingorani says.
