Orthotopic animal models provide the best fidelity for recapitulation of the tumor microenvironment, which are invaluable for cancer and drug development studies [ 16– 23].
Accepting the pivotal role of yolk sac as the single origin of adult-type hematopoiesis is the key for developing the recapitulation strategy for efficient production of dHSC in well-defined artificial microenvironment.
For Site2 targeting, NSB1 cells were used, which were used for recapitulation of MYC/IgH translocation (Fig. 2A D) and harbor Site1 at IGHA2 locus.
His note-for-note recapitulation of the love music marks the point at which he chooses the mirage over life itself.
In some cases, such as the ventricular IKs channel complex, it is well established that the KCNQ1 α subunit must coassemble with the MinK (KCNE1) single-transmembrane domain ancillary subunit for recapitulation of the characteristic, unusually slowly-activating IKs current.
Using expanded, redifferentiated costochondral cells in the self-assembling process allows for recapitulation of robust mechanical properties, and induced SZP expression, key characteristics of functional articular cartilage.
In 3D cysts of cultured Madin Darby canine kidney (MDCK) cells, a well-established system for recapitulation of epithelial tube morphogenesis, PI 4,5 P2 is a key determinant of the apical membrane, where it is highly enriched (Martin-Belmonte et al., 2007).
Additional evidence for recapitulation of the earliest kidney differentiation stages in WT has been provided by molecular studies, exemplified by the WT1, IGF2 and other genes, implicated in nephrogenesis and associated with Wilms' tumorigenesis.
For recapitulation of the ces-D mutant phenotypes, a vector was constructed that allowed constitutive overexpression of CES under control of the 35S promoter in plants and conferred resistance to the antibiotic gentamycin.
Expression of rBDNF-lacZ mRNA in the heart of rBDNF-lacZ-BAC transgenic mice reported here (with 144 kb region 3′ of the rBDNF contains regulatory elements necessary for recapitulation of endogenous BDNF expression in the brain, heart, and lung, indicating that regulatory elements governing BDNF mRNA expression in these tissues are located within the 207 kb rat genomic sequence of the transgene.
