A striking observation is that the aberrant expression of PRL and PROK1 in timed endometrial biopsies from RPL patients is recapitulated upon differentiation of purified ESCs maintained in prolonged cultures.
These changes were not recapitulated upon siRNA targeting of BCAR1.
In one study using human ES cells, it was suggested that imprinted XCI can be recapitulated upon differentiation towards the extra-embryonic lineages (Dhara and Benvenisty 2004).
Moreover, also the cooperative effect of Wnt and BMP signaling during early hematopoietic development could be recapitulated upon in vitro differentiation (Wang & Nakayama, 2009).
Surprisingly, however, we noted that these changes were not recapitulated upon treatment with siRNA targeting the proposed TNK2 downstream effector, BCAR1.
For example, Freire et al. have measured the anti-EGFR monoclonal antibody using DSC and showed that the data from an initial thermal denaturation were not recapitulated upon subsequent re-scanning [3].
99– 101 On the contrary, the endogenous function of a selected miRNA can be recapitulated upon introduction into the target cells of synthetic miRNA mimics consisting of a "guide strand," which is identical to that of the selected miRNA and a "passenger strand," which is chemically linked to a "carrier" molecule, such as cholesterol, for facilitating cellular uptake.
Metastases were detected in the peritoneal and thoracic cavity, contralateral mammary glands, lungs and bone (data not shown), illustrating that the full metastatic spectrum of human ILC is recapitulated upon orthotopic transplantation of mILC cell lines derived from the Wcre Cdh1 F;Trp53 F model.
Therefore, p53 protein was preferentially expressed in the proliferating tissue compartment upon induction, essentially recapitulating its gene expression pattern.
Genetically engineered mouse (GEM) models of cancer have progressively improved in technical sophistication and accurately recapitulating the cognate human condition and have had a measurable impact upon our knowledge of tumorigenesis.
