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Manual placement and rebuilding of a model for the mDia1 bridge element using the previously determined structure of the core FH2 domain of mDia1 [41] (PDB ID 1V9D) were performed in the program O [53].
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Refinement was carried out with PHENIX (version 1.6) [46] with iterative rebuilding of the model using Coot [47].
Several rounds of refinement were performed, alternating with manual rebuilding of the model after visualization of 2|Fo| – |Fc| and |Fo| – |Fc| maps using programs O [51] and COOT [52].
Subsequently, manual rebuilding of this model was performed with COOT, with reference to SIGMAA-weighted [61] 2mFobs – DFcalc and mFobs – DFcalc Fourier electron density maps, and this was alternated with restrained refinement with REFMAC5 using one TLS domain per protein chain.
Contrary to the use of ensembles of crop models, we endorse that efforts be concentrated on selecting or rebuilding a model that includes approaches that better describe the agronomic conditions of the regions in which they will be applied.
VIDEO: Watch now Mutombo Hospital, opened in 2007, aims to be a model for the rebuilding of a national medical system in DR Congo, where the collapse of the health care infrastructure has left millions vulnerable to a multitude of illnesses that are easily preventable in much of the developed world.
With the legendarily affordable real estate and without needing to pay for car payments, gas or insurance, bicyclists could rebuild Detroit into a model of a two-wheeled economy.
Her film about a teenager who was modelling the rebuilding of a dream Aleppo with cardboard, glue and bits of rubbish showed a rare scene of hope from within that shattered city.
Xfit [29] was used for graphical map inspection and manual rebuilding of the atomic model.
It's been devastation that requires major rebuilding of business models and valuation benchmarks.
The fourth paper covers a comparison of digitally recorded and Additively Manufactured dental casts with original plaster cast study models; useful for digital archiving and subsequent rebuilding of study models.
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