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Two pan-BTV RNA positive spleen fragments, sampled at the end of hunting season in 2008, were reassessed by using a BTV-8 genotype specific RT-qPCR (4 ); results were positive for all.
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The geocoding was then reassessed by us for accuracy and completeness.
The purity of T cell elution was reassessed by flow cytometry using primary labeled monoclonal antibodies against CD4 and CD8.
The efficiency of T-cell depletion was reassessed by flow cytometry using a cocktail of primary labelled mAb against the T-cell markers listed above.
Two hundred consecutive film sets were assessed by symptomatic mammographers and 22% were reassessed by a screening mammographer, using Perfect, Good, Moderate, Inadequate (PGMI) criteria [ 2].
All tumour specimens were reclassified on hematoxylin-and-eosin-stained slides, and histological type and tumour grade were reassessed by a pathologist (PM) using standard diagnostic criteria.
One year later, the soldier was reassessed for persisting antibodies by using the same ELISA and for Leishmania DNA in a blood sample stored in EDTA by using the Leishmania OligoC-Test (Coris BioConcept, Gembloux, Belgium).
This approach has been recently reassessed by Mozzafari et al. [22] who used the aspect ratio of face ellipse as geometric feature to reinforce appearance information.
Coagulation status was assessed using PT, and the status was reassessed by thromboelastometry for prolonged PT.
63 After 5 years of use, the risk of fractures should be reassessed by clinical history, radiography, and bone densitometry to define the best approach to future treatment.
Investigation of inter- and intra-examiner reproducibility and repeatability were carried out using a computer generated randomisation table to select 10% of individual radiographs, which were then reassessed by two of the authors (DE and NI).
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