Reassembly yielded a single contig for M. austroriparius Cluster 1 that was identified as the LINE element L1MAB_ML in RepBase.
How might this reassembly take place?
As in the first scenario, this would lead to a reduction of the CtxB monomer capable of associating (deprotonated CtxB monomer) at low pH and to a lower yield of reassembly.
Thus, looking at the effect of the toxin concentration on the yield of reassembly for different pH conditions, would allow discriminating the scenari 1&2 from the scenario 3. The effect of the toxin concentration on the toxin reassembly was tested over the pH range 5.0 to 8.0.
The latter, they found, yielded on reassembly a sequence essentially identical to the original; the other two gave a fairly nonsensical jumble of DNA.
The yield of the reassembly of CtxB was plotted against pH (Fig. 1B).
The enhanced version can also route variable-length packets without doing packet reassembly at the output.
The yields of toxin reassembly monitored by GM1-Elisa at 35 µM were plotted against pH similarly to what had been performed at 8.6 µM (Fig. 5A).
The yields of CtxB reassembly increased with increasing pH and described a sigmoid curve with an inflexion point around pH 6.0.
If protonated CtxB monomers misfold, the amount of deprotonated CtxB monomer which remains available decreases at low pH, leading to a reduction in the yield of the toxin reassembly.
