Sentence examples for reassembly step from inspiring English sources

The peptide library is generated by random digestion of the gene encoding the target viral protein, followed by a PCR-based reassembly step that results in fragments with controllable lengths (normally in the range of 100 500 bp) [24].

This reassembly step was required to ensure that all relevant ESTs were included as our experience with Cap3 and the large soybean EST collection showed that the Cap3 assemblies were often incomplete.

A protein called Prp3 is known to be involved in these assembly, disassembly and reassembly steps.

Charges of CP and CP-chimera were calculated using Protein Calculator v3.3, (C. Putnam, The Scripps Research Institute, USA) at pH 7.4 and 4.8, corresponding to buffer conditions reflecting the CCMV CP disassembly and CCMV VLP reassembly steps as described above.

To find out whether encapsulation of RNA could be correlated to epitope sequences with a positive charge, similar to the original N-terminus of CP, the charges of the N-terminal domain from the CCMV CP-derived chimera were calculated (see Materials and Methods) at pH 7.4 and 4.8, corresponding to buffer conditions reflecting the CCMV CP disassembly and CCMV VLP reassembly steps.

The protocol is somewhat skill-intensive, involving the fragmentation of the genes to be shuffled with DNAseI and a long, primerless reassembly PCR step.

Stop reassembly at step 5. Put the brass washer in this exact position when reinstalling the shot counter.

This indicated that increasing the toxin concentration promoted the reassembly by allowing the formation of CtxB oligomeric species, step particularly inhibited at low toxin concentration.

Based on our findings, we hypothesize that reincorporation of HEXIM1 into the 7SK snRNP is likely the regulated step of reassembly of the 7SK snRNP containing P-TEFb.

It could be envisioned, for example, that yFACT-mediated nucleosome reassembly is a required step in ensuring proper disengagement of yFACT from the ends of genes and mutations in the histones or conceivably in other proteins as well that interfere with this step would cause accumulation of yFACT at the 3′ ends of genes.

The soluble CCMV CP fraction was finally reassembled into VLPs during 2 dialysis steps against reassembly buffer (0.1 M NaAc, 0.9 M NaCl, 0.010 M MgCl2 pH 4.8) and concentrated by ultracentrifugation (Beckman) on a 30%% sucrose cushion at 40,000 rpm for 3 hours.