Sentence examples for reasonable sequencing depth from inspiring English sources
The remaining sequences represented the small RNA population, which accounted for 83% of reads (Table 1), suggesting a useful set of sRNAs with reasonable sequencing depth.
Sequencing the fragments bound to the MBD2 domain provides a genome-wide view of methylation patterns at reasonable sequencing depths.
To achieve the required sequencing depth at a reasonable cost, we used target enrichment to sequence SNP markers and developed a statistical framework based on model selection to estimate intervals of mapped QTL peak locations.
We were able to show that for datasets of biologically reasonable size (i.e. 30 X sequencing depth for human genome) Hadoop is comparable to HPC in terms of CPU-hours and is becoming an attractive option for bioinformatics data analysis from an e-infrastructure perspective.
Based on previous studies of complex plant genomes (e.g., barley and pea), we scaled our data collection efforts to produce ∼1% genomic coverage (i.e., 0.01× of the genome at 1× depth), as this sequencing depth has been shown to yield reasonable summaries of TE abundance for elements present at ≥1,000 copies/genome (Macas et al. 2007; Wicker et al. 2009).
(B) Sequencing depth distribution.
The results also showed that increase in sequencing depth has no effect on the sequence alignment.
Results were adjusted for sequencing depth by converting to counts per million sequences detected (cpm).
Instead, a reasonable approach is to minimize the amount of missing data by sequencing fewer sites at a higher target coverage, taking into account the variable sequencing depth that will be observed.
TG performed sequencing depth simulations.
