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The relatively small number of PDZ domain genes in the Drosophila genome makes it a reasonable pool of candidates to test comprehensively for their role in border cell migration.
The initial sample size was based on an estimate of the proportion likely to respond to a community survey, to give a reasonable pool of patients to purposefully sample a heterogenous sample for interviews.
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It was therefore reasonable to pool the two aCGH datasets (GSE19180 and GSE 9654) for further pathway analysis.
Although there was some heterogeneity between the questionnaire subgroups (P value = 0.11) (Analysis 14.1), it did not seem reasonable to pool the results based on such different interventions.
Although there is only some heterogeneity between the questionnaire subgroups (p=0.11; figure 3), it did not seem reasonable to pool the results based on such different interventions.
This suggests that our results are not driven by a specificity of one of the two sub-groups and that it was reasonable to pool them for the discovery analysis.
As no valid definition of cognitive impairment has been used, and given the finding of differential effects of other anti-dementia drugs on dementia and cognitive impairment [ 9], it appears no longer reasonable to pool these two indications.
The results of this conversion lead us to believe that the exposure levels across the two groups of study participants cover a range of values along the distribution of MeHg exposure in adults, and therefore it is reasonable to pool the results.
Pooling multiple BACs together reduces the number of libraries required and, with reasonable size pools, keeps assembly complexity sufficiently manageable.
These results were reasonable, as the pool of UDP-GlcNAc was increased by the inhibition of tunicamycin, so more was available for O-GlcNAcylation.
Obviously, reasonable increase of pool coverage will considerably reduce F- hits and increase the success rate and precision of clone deconvolution.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com