Sentence examples for reasonable expression levels from inspiring English sources
The target's surface expression has to be stable under various experimental conditions with reasonable expression levels for effective barcoding.
Both the trait genes and selection markers seemed to have reasonable expression levels, and some reads from papaya also showed some diversity in the 5' end of the coat protein transcript.
The entire 5'-sequence of the fuc-t gene, which encompasses 2087 bp, induces reasonable expression levels of the luciferase reporter gene at 194% compared to the CaMV 35S promoter.
Further, it became obvious that for reasonable expression levels, freshly transformed cells, mid-log precultures, and high levels of ampicillin (200 μg/ml) were essential to maintain construct stability.
We extracted expression profiles for all microarray elements, showing reasonable expression levels some time during leaf development, and performed a hierarchical clustering on the expression profiles (see Additional file 1).
Due to the ability to perform in vivo at biologically reasonable expression levels, as well as the ability to detect protein complexes with fewer false-positives [ 6], AP-MS approaches have become increasingly popular, although their throughput is lower than Y2 H approaches.
For the transient transfection approach, it is relative easier to reach to a reasonable protein expression level, but this expression level may vary from batch to batch.
Overall, the correlation illustrates reasonable concordance in basal gene expression levels between the two models.
In addition to exploring miRNA activity, our study addresses the question of whether miRNA expression levels are reasonable surrogates for activity in sarcomas.
However, it has been shown that proteins repressed by more than 30% also tend to destabilize at the transcript level [53] – meaning that examination of expression levels is a reasonable surrogate for large translational repressions.
However, assuming that these effects do not produce strong biases, the piRNA read counts should give a reasonable estimate of the piRNA cluster expression levels.
