Both the trait genes and selection markers seemed to have reasonable expression levels, and some reads from papaya also showed some diversity in the 5' end of the coat protein transcript.
Various IPTG concentrations were tested; mild in-duction of E. coli M15/pQE32-8 using 10 μM concentrations of IPTG leading to only partial clearing of the promoter turned out to be a good compromise between toxicity and reasonable expression yields.
We extracted expression profiles for all microarray elements, showing reasonable expression levels some time during leaf development, and performed a hierarchical clustering on the expression profiles (see Additional file 1).
Further, it became obvious that for reasonable expression levels, freshly transformed cells, mid-log precultures, and high levels of ampicillin (200 μg/ml) were essential to maintain construct stability.
According to the deep self concordance model, then, the asymmetry in attributions is a reasonable expression of the folk psychological distinction between the deep and shallow self.
Therefore, a reasonable expression for is as follows: (13) where V max and are additional model parameters.
While Rosin is banning herself from bitching and moaning, she explains, she permits herself the reasonable expression of genuinely negative circumstances in a clinical, straightforward tone.
That seems a reasonable expression of the principle that balances the rights of council-tax payers generally and democratic decisions about causes that local people want to support.
To aspire to such a responsible, reasonable expression of diversity in our societies, we must explain, educate, learn to know one another and to know and respect our neighbours.
The minister said this call from Gooda was a reasonable expression of his outrage but he said Giles had been devastated by the Four Corners report and was now rolling up his sleeves.
