For this reason, the understanding of gene expression profiles, which provide signatures of all the active genes and their interconnections in a cell, contain valuable information about the functioning of key pathways, as expressed by the hallmarks of cancer and, hence, enable a practical investigation of functional mechanisms thereof [ 28- 36].
Our current understanding of the cytosolic signaling pathways of these fusion proteins alone cannot explain the basis for these differences, and we reasoned that examination of gene expression changes regulated by the three fusions might provide insights in this regard.
Another reason for this variation of gene expression could be that under control conditions most CRKs were expressed at very low levels; consequently, a minor perturbation either by genetic mutation or growth condition could lead to altered expression.
The combination of co-acting transcription factors might be one reason for the level of gene expression being either induced or repressed.
For these reasons we limited analysis of gene expression profiles only for putative cytoplasmic proteins.
We reasoned that the hierarchy of gene expression correlations would allow a ranking of immune checkpoint/modulatory gene expression linked to the IFNγ-STAT1-IRF1 polaresponsesponse in DLBCL.
Because differentially expressed genes were identified by class comparison prior to the exercise challenge, we reasoned that a comparison of gene expression in CFS and control subjects, using genes categorized by ontology, would more efficiently reveal perturbed physiological pathways.
Another reason is that the signatures of gene expression difference between normal and cancer obtained in different studies are not sufficiently reproducible or informative to be prognostically useful, although they do give valuable insights into the pathogenesis and biology of human tumor metastasis [ 4].
One of the reasons is that the regulation of gene expression is decoupled from protein translation.
Thus, there is a good reason for hypothesising that epigenetic modification of gene expression plays a role in the development of early mother-infant relationship, even if at present it seems impossible to study these processes in human infants.
