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As a result, fingers closing will be kinetically invisible and rate-limited by the DNA rearrangement step.
We therefore favor the alternative explanation that fingers closing is fast but the DNA rearrangement step is required before fingers closing can take place.
However, it is possible that the temperature-sensitive reaction is merely a conformational rearrangement step, which is not relevant to any catalytic step.
In our simulations of geometric deletion events, we 'marked' some duplicate genes for deletion before carrying out the rearrangement step, and only actually deleted them afterward.
For 5-LOX, the magnitude of the normal SIE for Vmax[AA], with and without ATP added, indicates a hydrogen bond rearrangement step (i.e., general conformational change), similar to that seen for other LOX isozymes.
This suggests that the amine is integral to the mechanism of epimerization, with the reaction mechanism in Supplementary Data Section S1 Scheme 1 explaining the observation if the Amadori rearrangement (Step 2) is significantly reversible.
Similar(51)
The genome rearrangement steps were predicted by algorithms implanted in CREx server [ 88] together with gene rearrangement models reported in previous arthropod mitochondrial genomes [ 56].
The scheme includes rearrangement steps (k3 and k7) and hydrogen atom abstraction steps (k5 and k11) for both hydroperoxidation and epoxidation, respectively.
Their scenario implies that an identical series of 15 rearrangement steps happened convergently in both K. waltii and A. gossypii after they diverged from their common ancestor.
Because current cluster structure is a product of multiple DNA rearrangement steps, elements functional in the past may no longer be located at cluster termini.
Finally, these two processes require different substrate rearrangement steps to abstract a hydrogen atom from the substrate and donate it back to the intermediate.
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