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Moreover, it was not rearing frequency that predicted conditioned freezing in our rats, but rather rearing duration: the average length of a single rearing episode.
Rearing frequency, total distance moved, and total duration movement were monitored for 30 minutes, with data presented as cumulative total values of each parameter.
TTC9A−/− animals consistently showed anxiety in the open-field test with increased immobility duration and time-spent in the corner zone (C,F); as well as increased immobility and reduced rearing frequency in the novel-cage exploration test (G,H).
Rearing frequency was transiently impaired in female but improved in male R6/2 mice (see legend of figure 3 for statistical details).
Two validated methods were adopted for evaluating the neurobehavioral development [ 19] of pups: latency for uprightness and open field assay (ambulation, rearing frequency, cleaning activity and immobility time).
As shown in Figure 2C, compared to the control group, the NS group displayed a significant decrease (P < 0.05) in rearing frequency.
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Moving distances and durations, and rearing frequencies were calculated.
However, there is no significant difference in rearing frequencies between the groups (Fig. 1b).
The times of score locomotion and rearing frequencies, and the seconds of grooming time were measured during the OFT at the end of the CUMS procedure.
The score locomotion (number of line crossings), rearing frequencies (number of times an animal stood on its hind legs) and grooming time were recorded.
Hand-operated counters were employed to score the following behavioral parameters: locomotion (number of line crossings), rearing frequencies (number of times seen standing on hind legs), and number of defecations within 5 min.
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