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Horizontal locomotion and rearing activity were evaluated for 4 hours per month.
Other behaviors were normal in Pax6 rats, except for rearing activity and the light-dark choice test (data not shown).
Open-field testing was performed at 2.5 and 4 months post-injection to measure locomotor (Fig. 6B) and rearing activity (Fig. 6C).
The negative influence of human density on habitat suitability could be related to increasing disturbances and/or to a low livestock rearing activity in highly human populated areas.
Taken altogether, it is reasonable to assume that impaired rearing activity in rSey2/+ rats may not be related to increased anxiety or depressed state but rather to a less exploratory phenotype.
However rearing activity was significantly lower in rSey2/+ rats than in WT (two-way analysis of variance (ANOVA) with repeated measures (genotype × time); main effect of factor 'genotype': F(1) = 6.54, P<0.02; Fig. 1D).
In the present study, we further examined behaviors of rSey2/+ rats and revealed that they exhibited abnormality in social interaction (more aggression and withdrawal) in addition to impairment in rearing activity and in fear-conditioned memory.
The number of photocell interruptions was collected by a computer and the following variables were recorded and analysed: horizontal activity (Ha, total number of beam breakings), locomotion (L, interruptions of photocells in the lower rows when there is a new beam broken, i.e. the animal has made an actual transfer) and rearing activity (Ra, all interruptions of photocells in the upper rows).
Meanwhile, vitamin A depletion mice showed hypersensitivity of rearing activity to MK-801.
Post hoc analysis evidenced that none of the drugs studied significantly affected the rearing activity.
Although rearing activity was correlated with ventricular enlargement, the overall association was not statistically significant.
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