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Whenever necessary, as in case of subdivided populations, realignment of the sequence was carried out using clustalX.
Realignment of the sequence from the merged locus with the region surrounding these gaps allowed us to fill in all of them.
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Results include the native FlowerPower subfamily-HMM based alignment and a realignment of the sequences using MUSCLE.
Upon assembly, the Applied Biosystems and Illumina realignments of the B6 sequence reads differed from the B6 reference sequence for 41 and 60 residues (Table 1), respectively; 29 of these differences were consistent in both datasets when compared to the reference sequence.
Thus, it is extremely unlikely that strain or template inconsistency contributed to the discrepancies in the realignment of our sequence to the B6 reference sequence.
A further realignment of the representative conserved sequences of each of the cluster revealed a DNA stretch of 1278 nts to be common to all.
After realignment of the trimmed, grouped sequences, custom perl scripts (available upon request) parsed alignments to generate figures and tally substitutions.
The crucial link that allows a productive iteration of motif modeling and realignment is the comparison of the sequence variations for each putative pair with the corresponding isostericity matrix to determine which basepairs are consistent both with the sequence and the geometrical data.
set in motion a realignment of the two parties.
It's a tectonic realignment of the music business.
And they have argued for a realignment of the left.
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