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To further increase the local realignment accuracy, after local realignment, we performed base quality score recalibration on the realigned BAM files using GATK's recalibration tool.
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Local realignment around indels (RealignerTargetCreator, IndelRealigner) and base score recalibration (BaseRecalibrator) were applied by GATK (The Genome Analysis ToolKit, version 3.1-1) (McKetnalet al., 2010) to ensure accuracy in identifying indels and single nucleotide variants (SNVs).
According to the locating results of clean reads among the reference genome, duplicate reads were removed using the Picard tool (http://sourceforge.net/projects/picard/), and GATK software (McKenna et al. 2010) was used to perform the local realignment and base recalibration to insure the accuracy of the SNP detecting.
SHEAR's local realignment component also helps to improve the accuracy of heterogeneity estimation by fixing incorrectly soft-clipped reads.
For the steps after read mapping, including marking duplication, realignment and recalibration, the relative contribution of each step to the accuracy of variant calling depends on the sequencing depth.
The local realignment component of SHEAR is shown to fix errant soft-clipping and thus improve the accuracy and confidence of SV predictions in comparison with running CREST by itself, as well as improve the accuracy of our heterogeneity estimations.
The realignment has paid dividends.
"I think it's probably a realignment".
Tiller blamed the rush of conference realignment.
"Well, I'm thinking about conference realignment".
So fell another domino in conference realignment.
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