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Local tidal conditions and inadequate drainage at this realignment trial contributed to significant channel erosion due to the establishment of water surface slopes and pooling between the newly realigned site and the adjacent intertidal zone.
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Using Genome Analysis Toolkit (GATK), reads were realigned around insertion/deletion sites and base quality scores were recalibrated.
Every read around the target site is realigned against the putative alternative gapped haplotypes.
RNA synthesis resumes when the polymerase diffusively realigns its active site with the 3′-end of the transcript.
All sampled sequences were first aligned using MAFFT (E-INS-I, version 6), manually realigned, and then ambiguously aligned sites at the boundary of ORF2 and 3′-UTR were excluded from the alignment.
Reads from both strands at each site were extracted and realigned with the genome and the proportion finding a unique best alignment recorded.
Therefore, we investigate if some of the candidate indel RNA-DNA differences are signatures of paralog sequences in the genome, by realigning the indel sites and surrounding sequences back to the entire reference genome (NCBI build 37) using BLAST [ 34].
All metal debris were carefully removed from the operating site, and the nasal mucosal flap was realigned and sutured using 4-0 Vicryl to provide a watertight mucosal seal.
In this example, the femoral neck is realigned into valgus to achieve compressive properties at the fracture site.
Three steps were followed to call InDels: 1) reads were realigned with SOAP2 allowing gaps; 2) considering the supporting reads for each site, at least one individual InDel existed in the population; 3) allotted InDels back to each individual.
Sequences of each of the 11 ribosomal protein genes were aligned individually using default settings in Muscle 3.8 (Edgar, 2004) and were realigned by predicted translation; alignments were manually refined and stop-codon sites removed.
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