Sentence examples for real-time master from inspiring English sources
The hope of Dashboard is that data acquired by volunteers from voters canvassing in Ohio will immediately be synced with that gathered by those running phonebanks in New Hampshire and with the outreach efforts of volunteers at myBarackObama.com, giving campaign bosses a real-time master view of the president's re-election efforts throughout the country.
The target gene was amplified from cDNA using SYBR green real-time master mix (MJ Research Inc.,Waltham,MA), 1 mM each primer and 5 µl of the cDNA product in a total volume of 20 µl.
qPCR was carried out using the Quantitect real-time master mix (Qiagen) on a Bio-Rad CFX96 PCR machine.
The real-time master mix, for one reaction, comprised 5.0 µL of 2 × SSoFast EvaGreen Supermix (Bio-Rad), 1.4 µL DNase-free water, and 0.8 µL of 5 µM of each primer.
500 ng of RNA were reverse transcribed in a 20 µl reaction using Superscript II. 1 μl of cDNA was amplified using specific primers (Table 6) and SYBER green real-time master mix (Life Technologies).
The reaction mixture consisted of 2 μl of the diluted cDNA samples, 0.4 μl each of the forward and reverse primers (10 μM), 10 μl of the real-time master mix and 7.2 μl of the PCR-grade water for a final volume of 20 μl.
The qRT-PCR was performed in a final volume of 20 μl containing 10 μl of real time master mix, 1 μl of probe/primer mix, 4 μl of nuclease-free water, and 5 μl of diluted RNA.
In each reaction tube, 1.0 µL of cDNA and 7.5 µL of ddH2O were added to 16.5 µL of a master mix containing 12.5 µL of RT2 Real-Time SYBR Green/Rox PCR master mix (SuperArray Bioscience Corp., USA) along with 2 µL of the specific forward and reverse primers.
qRT-PCR was carried out using the real-time PCR master mix (SYBR green, TaKaRa) with the CFX96 machine (Bio-Rad) following the manufacturer's instructions.
The same year, FTL Games released the real-time Dungeon Master on the Atari ST, with a larger view and party of four characters to control, and dungeon exploration was never the same again.
To test if the AmpliWax®-seal would interfere with the PCR reaction, we took 1 µl of purified JCV (∼1800 copies/µl) from a CSF clinical sample and added to a JCV-specific real-time PCR master mix.
