It is, in short, a work of scholarship that borders on the obsessive; a work made slightly easier, you imagine, by Lewisohn's previous Beatles publication, The Beatles: 25 Years in the Life (1987), in which the author practically stalked the Fabs in real time, figuring out exactly where they all were, and what they were doing, on any given day.
A 2-dimensional emotion response space, adapted from Schubert [52], was presented visually so that participants could report emotional responses to the performances in real time (Figure 1).
Therefore, to demonstrate that ATP synthase could be the receptor for hsp60, recombinant hsp60 was immobilized onto the biosensor chip of a Biacore X system, ATP synthase injected into the cell flow and their interaction followed in real time (Figure 1D).
Real-time figures showing these transfections are shown in Supplementary Figures 2B-I, II and III.
The primers used for real-time PCR are listed in Table 4. Real-time figures of ERR β transfection are shown in Figure 2A.
During exercise sessions, the smartphone streamed GPRS data to a secure server enabling the ECG trace, heart rate, walking speed, elapsed distance and patient location to be viewed in real-time (Figure 3).
To address whether TRAIL mRNA induction paralleled the increase in TRAIL protein, RT-PCR (Figure 3B) and real time PCR (Figure 3C) were performed with total RNA extracted from transfected HT29 cells; TRAIL mRNA induction was noted with NFATc1 overexpression.
Microarray data were confirmed by real time PCR (Figure 5A).
An over-expression of at least 50-fold was confirmed by real time PCR (Figure 4A).
A 25-fold over-expression of miR-17 was verified by real time PCR (Figure 6A).
