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Samples were submitted to the Duke IGSP Genome Sequencing & Analysis Core Resource for real-time circular consensus sequencing using a Pacific Biosciences RS instrument and C2 chemistry.
The first one, ATLAS Beam Condition Monitor (BCM) utilizes fast front end amplifiers and processing electronics capable of handling events in real time and storing data in circular buffer thousand LHC turns deep for post-mortem analysis.
Sequencing by synthesis resulted in six large genome fragments, while the single molecule real time sequencing approach yielded one circular chromosome sequence.
Here we investigate the utility of Pacific Biosciences single molecule real-time (SMRT) circular consensus sequencing (CCS) as an alternative to traditional cloning and Sanger sequencing PCR amplicons for gene family characterization.
The data processing system was based on a circular buffer to read real time acceleration data.
As they spoke, this data was translated into a circular shape that grew outward in real time.
In this paper an exact analytical model for the chloride penetration in RC elements with circular cross-section is used to evaluate the real time to corrosion initiation of these RC elements.
While the sample was drying, they monitored the movement of PS particles in real time using an optical microscope, which revealed dynamic rearrangement of particles toward to the center region of the circular patterns.
To evaluate relative viral fitness, a quantitative real time PCR (QPCR) assay was developed to measure production of total HIV-1 DNA (extrachromosomal, integrated and 2-LTR circular forms) for a period of between 96 and 158 h post-infection.
(Real Time Economics) Funny.
"Not in real time.
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