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The expression pattern of key methanol metabolism genes, which may be responsible for formaldehyde degradation, was investigated further with the quantitative real-time chain reaction (qRT-PCR) method.
Real time polymerase chain reaction.
* RT-PCR Real time polymerase chain reaction.
qRT-PCR Quantitative Real Time Polymerase Chain Reaction.
Real time polymerase chain reaction (PCR) primers were custom synthesized by Integrated DNA Technologies IDTT), Coralville, IA, USA.
Cartilage-specific genes expression was measured by quantitative real time polymerase chain reaction.
β-globin real time polymerase chain reaction was performed using primers and hybridisation probes as described.
Microchimerism populations are tested by real time polymerase chain reaction (RT PCR).
Quantitative real time polymerase chain reactions were performed with a Roche Light Cycler 480 II.
The gene panel constituting the MHC II was quantified using q- real time polymerase chain reaction.
Trizol and the oligonucleotides for real time polymerase chain reaction (qPCR) were from Invitrogen (CA, USA).
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