To study the effects of CDDO-Me on Stat3 nuclear translocation in live cells, a real-time cell based assay was used as described below.
The real time cell electronic sensing assay is based on electrical impedance readings in cell monolayers plated in wells containing built-in gold electrodes.
In the following two label-free techniques were used, the impedance-based xCELLigence real time cell analyzer (RTCA) and the Cell-IQ Analyzer, based on automated microscopy.
Random cellular motility was studied using real time cell tracking.
To identify the interruption of IL-6 dependent Stat3 nuclear translocation by CDDO-Me, a novel real-time cell-based method was developed to image the EGFP-Stat3 chimera in the nucleus and cytoplasm in human osteosarcoma cell line U-2OS.
To investigate whether RRM2 plays a role in modulating cell adhesion in CRC, we developed a real-time cell adhesion assay based on the RT-CES® system.
The real-time cell electronic sensing assay based on electrical impedance readings in cell monolayers plated in wells containing built-in gold electrodes was performed.
The estimation is based on dynamic measurements generated by a real-time cell electronic sensor (RT-CES) and on existing cytotoxicity mathematical dynamic models.
The estimation is based on dynamic measurements generated by a real-time cell electronic sensor (RT-CES) and cytotoxicity dynamic models.
The migration and chemokinetic potential of the cells was measured using an xCELLigence System RTCA DP real-time cell analyser fitted with CIM plates (05665817001, Roche, UK), which is based on the Boyden chamber model [ 27].
