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For all these compounds, cytotoxicity was evaluated using sulforhodamine B assay and real time cell growth tracking, respectively.
Cytotoxicity and real time cell growth experiments showed no toxic effects of laser treated material on cells.
Real time cell analysis.
The cells were collected for Real time cell analysis (RTCA), Annexin V assay and Hoechst staining assay.
Random cellular motility was studied using real time cell tracking.
Real time cell analyses of proliferation, adhesion and migration were performed in triplicates on xCELLigence instruments (Roche, Hvidovre, Denmark).
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(H) Real-time cell proliferation analysis in the presence of miR-564 transfection or control miRNA.
In real-time cell cross-sectional area (Size [µm²]) and deformation were calculated and shown in scatter plot.
The present work examines, via real-time cell analysis, whether histamine and tyramine show synergistic toxicity towards intestinal cell cultures.
When encapsulated, VICs attained spread morphology, and proliferated and migrated as shown through real-time cell microscopy.
With the development of a real-time cell sorter, the researchers could now, for the first time, longitudinally collect CTCs from the same mouse.
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