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The sensitivity of each real time assay was established by comparing real time PCR to bacterial culture.
To confirm successful DNA extraction a human C reactive protein real time assay was run for each DNA extraction.
The real time assay sequences, cycling conditions and quality control process are described in the Supplementary Text S1 and Supplementary Figure S1.
Real Time assay oligos for these genes are described in Table 4.
The reproducibility of the real time assay was evaluated for initial DNA content, presence of PCR inhibitors etc.
FV, PS and IB developed the HIV-1 DNA real time assay and performed all the experimental work.
Similar(47)
This is a non-real time assay, which means key cellular events can be missed.
The real-time assay described is rapid, specific and sensitive.
Based on this, we describe a sensitive and confirmatory real-time assay for discriminating apoptosis and necrosis.
The multiplex real-time assay was at least 10 times more sensitive than conventional protocols for viroid and phytoplasma detection.
The real-time assay did not amplify sequences from the corresponding genomic regions of feline calicivirus (also in the genus Vesivirus) and representative members of the genus Norovirus.
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