Sentence examples for real time amplification from inspiring English sources

For quantitative real time amplification, SYBR green was used on a Biorad IQ5 system.

H2B was selected due to real time amplification efficiency (E = 1.973), lower Blast E-value, and lower coefficient of variation among replicates (CV = 0.154).

A master mixture of QNASBA (Quantitative Nucleic Acid Sequence Based Amplification) was used to quantify marker proteins by real time amplification of the aptamers.

This detection can be performed by real time amplification using the specific Not I probe (Figure 1) or following gel analysis after restriction by NotI.

Oligonucleotides 5'-TAAAGTGCTTATAGTGCAGGTAG-3' and 5'-CGCAAGGATGACACGCAAATTC-3' were used as forward primers respectively of miR-20a and U6 in the real time amplification mixtures.

However, the device can also be used together with a computer to generate real time amplification plots as the reaction progresses (as done in this study).

Each mutation was detected by real-time amplification followed by hybridization with two fluorescent probes designed with the sequence complementary to the wild-type sequences of gyr genes.

We developed a one-step, 3-h, reproducible, and accurate real-time amplification refractory mutation system which can simultaneously detect 28 ATP7B mutations.

With the increase in sensitivity of the detection methodology, there is an increasing concern about the interpretation of positive NoV results by real-time amplification.

A cohort of 346 lung cancers was tested with a commercial kit for EGFR mutations; nine of the cases had upward real-time amplification curves at late cycles.

A fluorescent dye, calcein, was used as an indicator for the endpoint visual detection and/or real-time amplification of HRSV RNA.