Assuming that the concentration of TF is a linear transformation of EA, we feed these simulated concentrations of the TFs into a chosen regulatory pattern described in Figure 1 and derive the expression pattern of the target gene (real_target_exp) according to equations 1 and 2. Noises (normal 0,1)) are added to all the real expression patterns for both TFs and the target gene.
From this EST database, we are currently identifying candidate genes for several horticulturally significant traits, such as cold hardiness, chilling requirement, fruit color, etc. based on predicted or real gene expression patterns in blueberry or other plants.
Comparing with results of real-time PCR, the expression pattern was almost the same with a few differences (Fig. 5a c).
By using quantitative Real Time-PCR, the gene expression pattern of DEFA 1-3 anDEFAFA 6 was analyzed in tissue of different stages of carcinogenesis, derived from colorectal cancer patients.
To complete this study, we examined the tissue-specific expression pattern using real-time PCR.
This result is consistent with a previous real-time analysis of the expression pattern of pPLAIIIδ [ 5].
For analysis of gene expression, standard PCR for control of gene expression or real-time PCR for the expression pattern analyses was done.
Eight genes (GALP, ITM2A, CTNNBIP1, WIF1, BMPR1B, CTNNAL1, LOC396850, CNN2) were selected to confirm the expression pattern by real-time RT-PCR.
Because cathepsin K was strongly expressed in murine knee joints and has an essential role in joint destruction, we investigated its expression pattern by real-time PCR (Fig. 2b) and found a significant downregulation at the mRNA level on day 1 but no alteration of expression at the mRNA level on the other days in the course of AIA.
To identify the function of CsPI in C. spicatus, we detected the expression pattern through quantitative real-time PCR.
