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SlowFade® Gold antifade reagent with DAPI (S36938, Life Technologies) was employed as mounting medium.
ProLong Gold anti-fade reagent with or without DAPI was used for mounting (all from Molecular Probes/Invitrogen).
Finally, coverslips were washed in PBS and mounted in mounting reagent with 4,6-diamidino-2-phenylindole (Invitrogen, P36931).
We found that the transfection experiment displaying least influence on the expression pattern shows the highest viability of cells (FuGENE 6 Transfection Reagent with plasmid without insert).
The reconstituted SPR bound the SPR agonist substance P, 125I-labeled with Bolton-Hunter reagent, with low affinity.
The protein content was determined using Bradford reagent with bovine albumin as standard.
Coverslips were mounted using ProLong® Gold antifade reagent with DAPI (Invitrogen, Cat.no.: P-36931).
Phenotyping was performed with BD Multitest 6-color TBNK reagent with BD Truecount tubes, (BD Biosciences).
Coverslides were mounted with Prolong Antifade reagent with DAPI (Molecular Probes, Eugene, OR).
Sections were mounted using SlowFade Gold antifade reagent with DAPI (Invitrogen).
Coverslips were washed in PBS and mounted with ProLong Gold anti fade reagent with DAPI (Invitrogen).
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